Proximity Ligation Assay:Article Title: Cellular-based method for determining the potency of defibrotide
Article Snippet: Vacuum medium filter sterilization unit (Sigma Aldrich, Milan, Italy?). .. Computer Programs Microsoft Excel 2003. (Microsoft Corporation, Redmond, Wash., USA) PLA 2 (Stegmann Systems GmbH, Germany) Cells Human Microvascular Endothelial Cell line (HMEC-1, CDC, Atlanta, GA, USA) Liver sinusoidal endothelial cell line (SK-HEP-1, ATCC, Manassas, VA, USA) Reagents and Chemicals Defibrotide (Gentium, Italy) 9-beta-D-arabinofuranosyl-2-fluoroadenine, analytical grade (Sigma Aldrich, Milan, Italy), referred to as fludarabine or F-ara in the Figures and Examples below Doxorubicin, analytical grade (Sigma Aldrich, Milan, Italy) Amphotericin B (Sigma-Aldrich, Milan, Italy) Dimethyl sulfoxide (DMSO) (Sigma-Aldrich, Milan, Italy) Gelatin, 2% in water, tissue culture grade (Sigma-Aldrich, Milan, Italy) Dulbecco's phosphate buffered saline (D-PBS) (Sigma-Aldrich, Milan, Italy) Ethanol Absolute (Sigma-Aldrich, Milan, Italy) Fetal Bovine Serum (FBS) (Sigma-Aldrich, Milan, Italy) Penicillin-Streptomycin 100× (Sigma-Aldrich, Milan, Italy) MTT (Sigma-Aldrich, Milan, Italy) CCK-8 (Sigma Aldrich, Milan, Italy) Trypan Blue (Sigma-Aldrich, Milan, Italy) Eagle's Minimum Essential Medium (EMEM) ATCC Number: 30-2003 (ATCC Manassas, VA, USA) Oligonucleotide (ACGT)n of about 17 Kda (Sigma Genosys, Milan, Italy) Oligonucleotide (AC)n of about 17 KDa (Sigma Genosys, Milan, Italy) Glutathione (Sigma-Aldrich, Milan, Italy) Human tissue plasminogen activator (tPA) (Sigma-Aldrich, Milan, Italy) Molecular biology grade water (Sigma-Aldrich, Milan, Italy) Preparation of Cell Growth Medium for SK-HEP-1 Cell growth medium for SK-HEP-1 was Eagle's Minimum Essential Medium (EMEM) supplemented with 10% (v/v) of foetal bovine serum (FBS), 1×Penicillin-Streptomycin and 1×Amphotericin B. ..
Saline:Article Title: Cellular-based method for determining the potency of defibrotide
Article Snippet: Vacuum medium filter sterilization unit (Sigma Aldrich, Milan, Italy?). .. Computer Programs Microsoft Excel 2003. (Microsoft Corporation, Redmond, Wash., USA) PLA 2 (Stegmann Systems GmbH, Germany) Cells Human Microvascular Endothelial Cell line (HMEC-1, CDC, Atlanta, GA, USA) Liver sinusoidal endothelial cell line (SK-HEP-1, ATCC, Manassas, VA, USA) Reagents and Chemicals Defibrotide (Gentium, Italy) 9-beta-D-arabinofuranosyl-2-fluoroadenine, analytical grade (Sigma Aldrich, Milan, Italy), referred to as fludarabine or F-ara in the Figures and Examples below Doxorubicin, analytical grade (Sigma Aldrich, Milan, Italy) Amphotericin B (Sigma-Aldrich, Milan, Italy) Dimethyl sulfoxide (DMSO) (Sigma-Aldrich, Milan, Italy) Gelatin, 2% in water, tissue culture grade (Sigma-Aldrich, Milan, Italy) Dulbecco's phosphate buffered saline (D-PBS) (Sigma-Aldrich, Milan, Italy) Ethanol Absolute (Sigma-Aldrich, Milan, Italy) Fetal Bovine Serum (FBS) (Sigma-Aldrich, Milan, Italy) Penicillin-Streptomycin 100× (Sigma-Aldrich, Milan, Italy) MTT (Sigma-Aldrich, Milan, Italy) CCK-8 (Sigma Aldrich, Milan, Italy) Trypan Blue (Sigma-Aldrich, Milan, Italy) Eagle's Minimum Essential Medium (EMEM) ATCC Number: 30-2003 (ATCC Manassas, VA, USA) Oligonucleotide (ACGT)n of about 17 Kda (Sigma Genosys, Milan, Italy) Oligonucleotide (AC)n of about 17 KDa (Sigma Genosys, Milan, Italy) Glutathione (Sigma-Aldrich, Milan, Italy) Human tissue plasminogen activator (tPA) (Sigma-Aldrich, Milan, Italy) Molecular biology grade water (Sigma-Aldrich, Milan, Italy) Preparation of Cell Growth Medium for SK-HEP-1 Cell growth medium for SK-HEP-1 was Eagle's Minimum Essential Medium (EMEM) supplemented with 10% (v/v) of foetal bovine serum (FBS), 1×Penicillin-Streptomycin and 1×Amphotericin B. ..
MTT Assay:Article Title: Cellular-based method for determining the potency of defibrotide
Article Snippet: Vacuum medium filter sterilization unit (Sigma Aldrich, Milan, Italy?). .. Computer Programs Microsoft Excel 2003. (Microsoft Corporation, Redmond, Wash., USA) PLA 2 (Stegmann Systems GmbH, Germany) Cells Human Microvascular Endothelial Cell line (HMEC-1, CDC, Atlanta, GA, USA) Liver sinusoidal endothelial cell line (SK-HEP-1, ATCC, Manassas, VA, USA) Reagents and Chemicals Defibrotide (Gentium, Italy) 9-beta-D-arabinofuranosyl-2-fluoroadenine, analytical grade (Sigma Aldrich, Milan, Italy), referred to as fludarabine or F-ara in the Figures and Examples below Doxorubicin, analytical grade (Sigma Aldrich, Milan, Italy) Amphotericin B (Sigma-Aldrich, Milan, Italy) Dimethyl sulfoxide (DMSO) (Sigma-Aldrich, Milan, Italy) Gelatin, 2% in water, tissue culture grade (Sigma-Aldrich, Milan, Italy) Dulbecco's phosphate buffered saline (D-PBS) (Sigma-Aldrich, Milan, Italy) Ethanol Absolute (Sigma-Aldrich, Milan, Italy) Fetal Bovine Serum (FBS) (Sigma-Aldrich, Milan, Italy) Penicillin-Streptomycin 100× (Sigma-Aldrich, Milan, Italy) MTT (Sigma-Aldrich, Milan, Italy) CCK-8 (Sigma Aldrich, Milan, Italy) Trypan Blue (Sigma-Aldrich, Milan, Italy) Eagle's Minimum Essential Medium (EMEM) ATCC Number: 30-2003 (ATCC Manassas, VA, USA) Oligonucleotide (ACGT)n of about 17 Kda (Sigma Genosys, Milan, Italy) Oligonucleotide (AC)n of about 17 KDa (Sigma Genosys, Milan, Italy) Glutathione (Sigma-Aldrich, Milan, Italy) Human tissue plasminogen activator (tPA) (Sigma-Aldrich, Milan, Italy) Molecular biology grade water (Sigma-Aldrich, Milan, Italy) Preparation of Cell Growth Medium for SK-HEP-1 Cell growth medium for SK-HEP-1 was Eagle's Minimum Essential Medium (EMEM) supplemented with 10% (v/v) of foetal bovine serum (FBS), 1×Penicillin-Streptomycin and 1×Amphotericin B. ..
CCK-8 Assay:Article Title: Cellular-based method for determining the potency of defibrotide
Article Snippet: Vacuum medium filter sterilization unit (Sigma Aldrich, Milan, Italy?). .. Computer Programs Microsoft Excel 2003. (Microsoft Corporation, Redmond, Wash., USA) PLA 2 (Stegmann Systems GmbH, Germany) Cells Human Microvascular Endothelial Cell line (HMEC-1, CDC, Atlanta, GA, USA) Liver sinusoidal endothelial cell line (SK-HEP-1, ATCC, Manassas, VA, USA) Reagents and Chemicals Defibrotide (Gentium, Italy) 9-beta-D-arabinofuranosyl-2-fluoroadenine, analytical grade (Sigma Aldrich, Milan, Italy), referred to as fludarabine or F-ara in the Figures and Examples below Doxorubicin, analytical grade (Sigma Aldrich, Milan, Italy) Amphotericin B (Sigma-Aldrich, Milan, Italy) Dimethyl sulfoxide (DMSO) (Sigma-Aldrich, Milan, Italy) Gelatin, 2% in water, tissue culture grade (Sigma-Aldrich, Milan, Italy) Dulbecco's phosphate buffered saline (D-PBS) (Sigma-Aldrich, Milan, Italy) Ethanol Absolute (Sigma-Aldrich, Milan, Italy) Fetal Bovine Serum (FBS) (Sigma-Aldrich, Milan, Italy) Penicillin-Streptomycin 100× (Sigma-Aldrich, Milan, Italy) MTT (Sigma-Aldrich, Milan, Italy) CCK-8 (Sigma Aldrich, Milan, Italy) Trypan Blue (Sigma-Aldrich, Milan, Italy) Eagle's Minimum Essential Medium (EMEM) ATCC Number: 30-2003 (ATCC Manassas, VA, USA) Oligonucleotide (ACGT)n of about 17 Kda (Sigma Genosys, Milan, Italy) Oligonucleotide (AC)n of about 17 KDa (Sigma Genosys, Milan, Italy) Glutathione (Sigma-Aldrich, Milan, Italy) Human tissue plasminogen activator (tPA) (Sigma-Aldrich, Milan, Italy) Molecular biology grade water (Sigma-Aldrich, Milan, Italy) Preparation of Cell Growth Medium for SK-HEP-1 Cell growth medium for SK-HEP-1 was Eagle's Minimum Essential Medium (EMEM) supplemented with 10% (v/v) of foetal bovine serum (FBS), 1×Penicillin-Streptomycin and 1×Amphotericin B. ..
|
ATCC is a verified supplier 
ATCC manufactures this product 